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Should I put MPG file to AVI lossless before applying any AviSynth script?

Hi,

I have an mpeg video (m2v/d2v) with YV12 colorspace and I'm wondering, should I open it in VDub and save it directly to AVI with Lagarith codec BEFORE applying any AviSynth scripts/effects on it?

Or can I just go ahead, open the d2v file, make some changes, and then save it to AVI?

I'm affraid to loss quality, it's an animation.

Any comment is welcome.

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